Turnover of proteoglycans in articular-cartilage cultures. Characterization of proteoglycans released into the medium
نویسندگان
چکیده
منابع مشابه
The heterogeneity of cartilage proteoglycans. Isolation of different types of proteoglycans from bovine articular cartilage.
Proteoglycans, proteins, and glycoproteins were extracted from femoral condyle (FC) and metacarpalphalangeal (MP) articular cartilages and fractionated by sequential density gradient sedimentation under dissociative conditions. The major high density proteoglycan (PG-I) accounted for 74% of the total proteoglycan extracted from the MP cartilage and 79% of that from the femoral condyle. These pr...
متن کاملChondrocyte-mediated depletion of articular cartilage proteoglycans in vitro.
The degradation of proteoglycan was examined in cultured slices of pig articular cartilage. Pig leucocyte catabolin (10 ng/ml) was used to stimulate the chondrocytes and induce a 4-fold increase in the rate of proteoglycan loss from the matrix for 4 days. Material in the medium of both control and depleted cultures was mostly a degradation product of the aggregating proteoglycan. It was recover...
متن کاملCartilage proteoglycans.
The predominant proteoglycan present in cartilage is the large chondroitin sulfate proteoglycan 'aggrecan'. Following its secretion, aggrecan self-assembles into a supramolecular structure with as many as 50 monomers bound to a filament of hyaluronan. Aggrecan serves a direct, primary role providing the osmotic resistance necessary for cartilage to resist compressive loads. Other proteoglycans ...
متن کاملDelayed aggregation of proteoglycans in adult human articular cartilage.
The biosynthesis and macromolecular organization of proteoglycans was studied in explants of adult human articular cartilage. In a series of pulse-chase experiments, labelling with (35S)sulphate, it was shown that the proteoglycan monomer is synthesized as a precursor that has a low affinity for hyaluronic acid. These findings suggest a possible mechanism by which the rate of incorporation of p...
متن کاملProteoglycans of Hyaline Cartilage
Proteoglycan monomers from guinea-pig costal cartilage, bovine nasal and bovine tracheal cartilage were observed in the electron microscope after being spread in a monomolecular layer with cytochrome c. The proteoglycan molecule appeared as an extended central core filament to which side-chain filaments were attached at various intervals. The molecules from the three sources displayed great ult...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1989
ISSN: 0264-6021,1470-8728
DOI: 10.1042/bj2590021